Expression, purification, crystallization and preliminary X-ray analysis of the EIIC^Glc domain of the Escherichia coli glucose transporter

The glucose-import system of Escherichia coli consists of a hydrophilic EIIA^Glc subunit and a transmembrane EIICB^Glc subunit. EIICB^Glc (UniProt P69786) contains two domains: the transmembrane EIIC^Glc domain (40.6 kDa) and the cytoplasmic EIIB^Glc domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICB^Glc subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIIC^Glc domain is described. A complete data set was collected to 4.5 Å resolution at 100 K.