Figure 2
Direct comparison of PSPC with XR-II + CCD. The solution scattering patterns from various protein solutions with both XR-II + CCD (solid lines) and PSPC (dotted lines) are shown. (a) Myoglobin (MW = 17.8 kDa). (b) Chicken egg albumin (MW = 45 kDa). (c) Bovine serum albumin (MW = 68 kDa). (d) Aldolase (MW = 158 kDa). All are dispersed in 10 mM HEPES (pH 7.0). Collection time was 10 s. For PSPC, Ar + CH4 gas was used. The incident beam was attenuated so that the rate of PSPC lies below 10 kcps in which the linearity was assured. The receiving slit before PSPC was 1 mm. The CCD area used for comparison was 0.9 mm × 50 mm. The data increment of PSPC was adjusted with a pixel size of XR-II + CCD. The small decrease in PSPC near the beam stop is due to optics alignment. |