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Figure 6
UV–Vis absorption spectra of cryocooled (100 K) cytochrome C thin film (top); vitamin B12 crystal (∼10 × 60 × 150 µm) (middle); and met myoglobin (metMb) crystal (∼10 × 80 × 150 µm) (bottom), crystallized as previously described (Beitlich et al., 2007BB6), taken using the on-axis spectrometer. The discontinuities observed at 470 and 765 nm are due to intense peaks in the xenon lamp spectra at these wavelengths. Data were collected with accumulation times of 10 × 100 µs, 5 × 20 µs and 4 × 0.8 ms, respectively, using a 10 µm slit width and a grating with 150 lines mm−1 in all cases.

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