Figure 5
Spectra measured from (a) native and (c) TU, (e) KF and (g) SAA soaked myoglobin crystals with increasing dose. The total dose absorbed by the crystals was around 6.5 MGy. Different shapes were observed for the time course curves of the peak at 566 nm: (b) native and (d) TU, (f) KF and (h) SAA soaked crystals with fits to the double exponential function with offset. The absorbance at 555 nm was subtracted from that at 566 nm to try to minimize the effects of solvated electrons. |