MxCuBE: a synchrotron beamline control environment customized for macromolecular crystallography experiments

Gabadinho, J.; Beteva, A.; Guijarro, M.; Rey-Bakaikoa, V.; Spruce, D.; Bowler, M.W.; Brockhauser, S.; Flot, D.; Gordon, E.J.; Hall, D.R.; Lavault, B.; McCarthy, A.A.; McCarthy, J.; Mitchell, E.; Monaco, S.; Mueller-Dieckmann, C.; Nurizzo, D.; Ravelli, R.B.G.; Thibault, X.; Walsh, M.A.; Leonard, G.A.; McSweeney, S.M.

doi:10.1107/S0909049510020005

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Figure 5
Analysis of X-ray-induced fluorescence (XRF) spectra in MxCuBE. (a) The spectrum from a crystal (see Fig. 2

) of a selenomethionyl derivative of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum (Tarbouriech et al., 2005

). The displayed spectrum is truncated so that peaks owing to Compton and Rayleigh scattering by the sample of the incident X-ray beam are not shown. Clicking on a peak in the spectrum allows identification of elements via a pop-up window. (b) The XRF spectrum from a crystal of thermolysin from Bacillus thermoproteolyticus (Mueller-Dieckmann et al., 2007

) showing the presence of chloride (Kα emission line, ∼2.6 keV), potassium (Kα, Kβ emission lines, ∼3.7 keV and ∼4.0 keV, respectively) and zinc (Kα, Kβ emission lines, ∼8.6 keV and ∼9.6 keV, respectively). The peak at ∼12.2 keV is due to Compton scattering by the sample of the incident X-ray beam. The Kα emission line of sulfur (∼2.3 keV) is also visible in this spectrum.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 17| Part 5| July 2010| Pages 700-707

doi:10.1107/S0909049510020005

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