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Figure 1
Crystals of HbGp obtained under two different conditions. (a) 10% PEG8000, 2.5 mM CaCl2, 50 mM Tris-HCl pH 7.5, which grew to approximate maximum dimensions of 0.1 mm. These crystals readily deteriorate after about a week (b) and often disappear altogether. Crystals obtained in the presence of 1.2 M sodium citrate, 2.5 mM CaCl2, 50 mM, Tris-HCl pH 7.5, can grow to a maximum dimension of almost 1 mm (c), but the best diffraction patterns were obtained from well formed crystals with typical dimensions of approximately 0.1 mm in all directions (d).

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