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Figure 2
Sequential centring of the sample holder (a–c) and the crystal (d–g). The loop centring is performed by applying an edge recognition method (a–b) followed by mask recognition and centre of mass calculation (c). The crystal is centred after UV illumination (d), contour recognition (e–f) and centring (g). The intersection at the red lines represents the beam centre. The blue dotted lines representing the boxes for centre of mass calculation were arbitrarily added for a better understanding of the steps in the centring process. The higher recognition contrast for the UV illuminated crystals is highlighted by comparing the centred crystal under normal light (h) and UV light (i), together with horizontal and vertical scans along the red lines.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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