Figure 3
Superposed structures in the active site of crystals soaking in PEA solution for 0 (control) (gray) and 15 min (magenta). Based on the Fo − Fc omit map contoured at 2.4 s, it was revealed that the product Schiff-base (TPQpsb) was formed in this crystal. The substrate channel of AGAO is shown by the (green) circle. The entrance of the substrate channel (green arrow) was closed by Phe105 and Leu358′ of a neighboring subunit that moved as a cap. |