Figure 1
(a) One representative negatively stained EM image of α-crustacyanin from the 251 total images used to extract 10021 α-crustayanin particles. Scale bar = 50 nm. (b) Four representative class-averages (1), the EM three-dimensional reconstruction calculated by angular reconstitution (2), and views of the final docked model to the EM map (3). Box size = 33.6 × 33.6 nm. The final dimensions of α-crustacyanin are 130 Å by 140 Å by 180 Å. The analysis program used was EMAN. |