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Figure 5
Molecular functional group peak area ratio. Height under the protein α-helix (∼1657 cm−1) bands divided by the height under the peaks of the β-sheet (∼1628 cm−1) bands at each pixel (pixel size 10 µm × 10 µm), representing the α-helix to β-sheet ratio in the sorghum seed tissue. (a) Visible image. (b) Spectra corresponding to the pixel at the cross hair in the visible image. (c) Chemical ratio image: α-helix (1657 cm−1) to β-sheet (1628 cm−1). (d) Region and baseline: α-helix (1657 cm−1) to β-sheet (1628 cm−1). (e) Three-dimensional image; peak height ratio of two peaks. (f) Ratio image profile set-up.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 18| Part 5| September 2011| Pages 790-801
https://doi.org/10.1107/S0909049511023727
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