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Figure 6
Molecular functional group peak area ratio. Height under the protein amide I (1650 cm−1) bands divided by the height under the peaks between ∼1180 and 950 cm−1 at each pixel (pixel size 10 µm × 10 µm) representing the protein to total carbohydrate ratio in the sorghum seed tissue. (a) Visible image. (b) Spectra corresponding to the pixel at the cross hair in the visible image. (c) Chemical ratio image: protein to carbohydrate ratio. (d) Region and baseline of protein amide I and II and carbohydrate. (e) Three-dimensional image; peak height ratio of two peaks. (f) Ratio image profile set-up.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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