view article

Figure 8
Left: structure of the rhodopsin dark state (blue) and the G-protein interacting Meta II state (red). The outward tilt of helix VI (H VI) opens a cleft on the cytosolic side that is a prerequisite for interaction with the G-protein. Right: FTIR difference spectrum of Meta II formation, calculated as Meta II minus rhodopsin absorbance spectrum. Negative bands (blue) occur owing to vibrational modes of the dark state while positive bands are caused by the illuminated state. Important spectral regions, indicative of protonated carbonyl, protein secondary structure alterations and chromophore isomerization are described.

Journal logoJOURNAL OF
ISSN: 1600-5775
Follow J. Synchrotron Rad.
Sign up for e-alerts
Follow J. Synchrotron Rad. on Twitter
Follow us on facebook
Sign up for RSS feeds