Figure 3
A data collection strategy from multiple spots on one crystal. On each spot of a crystal, a partial data set covering only a 15° rotation was collected to avoid severe radiation damage. The starting spindle position was staggered with a 5° increment on the next spot. Data from seven spots cover the maximum rotation of 45° for the plate scanner. The top of the figure shows the increased data completeness with data from each spot being added for a typical run of a BcGH3 crystal. The insert photograph shows an array of burned spots resulting from radiation damage after sequential data collection on them. |