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Figure 1
Evolution of radiation damage with absorbed dose on lysozyme crystals at room temperature monitored using different metrics. The dose D is defined as the total absorbed energy divided by the mass of the region of the crystal receiving nonzero dose (AD-ER), as defined by Zeldin et al. (2013BB61). Crystals were soaked at different uridine concentrations, from zero to 1 M, and diffraction data recorded at two different dose rates. (a) Decay of the mean normalized intensity I/I1, calculated in the 50–2 Å. resolution range. (b) Decay of the R-factor Rd with dose. Rd is calculated in the 50–2 Å resolution range. For clarity, error bars are not shown (data with error bars are plotted in Fig. S2 of the supporting information). (c) Evolution of ΔB as a function of dose. Lines show the fits of the experimental data using an exponential model [\Delta B] = [{B}_{0}[\exp(k\Delta{D})-1]]. Results of the fits are shown in Table 1[link].

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 24| Part 1| January 2017| Pages 53-62
https://doi.org/10.1107/S1600577516018452
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