Figure 5
Fitting of the experimental SAXS data (blue dots) with the theoretical scattering curve predicted from the crystal structure (PDB ID 4n8z, red line) for (a) lysozyme collected in static mode in the presence of 100 mM uridine (χ2 = 0.98) and (b) lysozyme without any additive collected by flowing the sample while exposed to the beam (χ2 = 1.08). Experimental data result from the averaging of the statistically similar frames below the critical dose validated by CorMap (Franke et al., 2015) (20 frames for lysozyme with uridine in static mode and two frames for lysozyme without additive in flow mode). |