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Figure 3
2FoFc electron density maps showing the CypA F113 side chain contoured at 1σ (blue) and 0.3σ (light blue) for increasingly damaged data. The least damaged model (in yellow) is shown together with the more damaged models for reference. The DWD for each model was (a) 0.016 MGy, (b) 0.038 MGy, (c) 0.06 MGy and (d) 0.124 MGy. As radiation damage progresses, the electron density for the minor conformer becomes less defined and the refined position of the alternate side chain moves closer to the major conformer.