Figure 5
Results of hydroxyl radical protein footprinting of FH. (a) Oxidation sites of factor H irradiated alone. (b) Oxidation sites of FH irradiated in the presence of C3b. The differences in oxidation are shown in orange circles. Factor H is represented as a grey surface. Red spheres indicate oxidized residues. PDB: 3GAV-1. (c) Representative dose-response plots showing the fraction of unmodified FH peptide as a function of X-ray exposure time (ms). [This figure has been adapted from one published in Biochem. J. (Baud et al., 2016).] |