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![[Figure 1]](fv5075fig1mag.jpg)
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Figure 1
Sketch of the scanning diffraction experiment on tissues. A 30 µm-thick tissue slice from an excised mouse heart, embedded in an agarose matrix, was mounted on a thin polypropylene foil and placed into the focus of the X-ray beam. Zoomed illustrations depict the highly regular arrangement of cardiac muscle fibres. Monochromated undulator radiation is focused down to micrometer spot size, using CRL transfocator optics. Scattered radiation is detected using a two-dimensional pixelated detector (here Eiger 4M, Dectris, Switzerland). An on-axis video microscope facilitates sample positioning and alignment with respect to the beam focus position. A circular beamstop housed in a helium-flushed flight tube blocks unscattered radiation downstream from the focus position. Finally, the sample is swept through the focus of the beam and diffraction patterns are recorded at regular intervals during scanning. The data are read and analyzed by a custom platform for automized data analysis for scanning diffraction experiments. RA, right artery; LA, left artery; AO, aorta; PA, pulmonary artery; LV, left ventricle; RV, right ventricle. |
![[Figure 1]](fv5075fig1.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
