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Figure 3
Radius distribution of pure freshly prepared lysozyme and BSA solutions as well as mixtures of lysozyme and BSA determined by in situ DLS. Solutions with different molar ratios (lysozyme:BSA) of both proteins were analysed by DLS for 10 s each and with up to 25-fold excess of lysozyme over BSA. Further, a highly polydisperse sample solution of aggregated BSA was measured after 1 month of storage at room temperature and is shown for comparison. A comparison of the radius distributions verifies the applicability of the in situ DLS instrument to identify and score inhomogeneities and impurities in sample solutions.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 25| Part 2| March 2018| Pages 361-372
https://doi.org/10.1107/S1600577517017568
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