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![[Figure 4]](co5097fig4mag.jpg)
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Figure 4
DLS experiments analysing samples in flow. (a) A suspension of latex microspheres (RH = 10.5 ± 0.5 nm, particle density 20.6 mg cm−3) was injected into a flexible tube attached to the capillary and maintained at a constant pump-driven velocity of 400 µl min−1 (red graph) to investigate the influence of the sample flow on the determined particle radius. After reaching the maximum intensity of light scattered by the microspheres, the pump was stopped for approximately 5 min. Finally, the pump was switched on again at the original flow rate of 400 µl min−1 (red graph) and the sample was washed out of the capillary. A hydrodynamic radius of 10.5 ± 0.7 nm was determined for the latex particles after averaging of the DLS data. The diameter of the blue spheres represents the relative abundance of the detected particle radii weighted by scattering intensities in arbitrary units. (b) BSA (9 mg ml−1; 66 kDa) and lysozyme (16 mg ml−1; 14 kDa) were analysed in a circular flow tubing applying the four-channel in situ DLS instrument to systematically follow the radius determination at varying flow rates. |
![[Figure 4]](co5097fig4.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
