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![[Figure 5]](gm5051fig5mag.jpg)
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Figure 5
Density maps calculated from the ICA components for thaumatin. (a) The first component of ICA for all eight disulfide bridges of thaumatin. The map was contoured at ±5σ and corresponds to the (dρ/dD) component of the specific radiation-induced changes. The red colour represents loss of electron density, while the green colour represents gain of electron density. (b) The second component of the ICA for all disulfide bridges. The map was contoured at +1.5σ and coloured blue because it represents a 2mFo − DFc-type map corresponding to the thaumatin structure with reduced disulfide bridges. Therefore, this component corresponds to large dose, as indicated also by Fig. 4(b) ![[link]](../../../../../../logos/arrows/s_arr.gif) . (c) The third component of the ICA. The map was contoured at ±4σ and corresponds to the non-linear with dose (d2ρ/dD2) component of the specific radiation-induced changes. The highest non-linearity was expected for the disulfide bridges undergoing the fastest initial damage, described in the map of the first component and in Table 3. Therefore, the colouring scheme for the map of the third component was selected so that magenta shows higher deceleration in the changes of electron density with dose, as described by the first component (a), while dark green shows slower deceleration in the changes of electron density with dose, as described by the first component (a). At larger doses, it is expected that the map of the third component would correspond to the purer (d2ρ/dD2) map; however, because ICA did not provide a fully independent component, this map represents a mixture of a small contribution from (dρ/dD) and a large contribution from (d2ρ/dD2). |
![[Figure 5]](gm5051fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
