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Figure 2
(a) Fluence to identify a protein with different requirements and assumptions. (i) Isolated protein in single 10 nm pixel and Rose criteria of 5 (Howells et al., 2009BB26). (ii) Protein in water, single 10 nm pixel (Howells et al., 2009BB26). (iii) As (ii) but within a field of view (FOV) for CDI of 500 nm (Villanueva-Perez et al., 2016BB67). (iv) As (ii) with a 31.6 nm voxel. (v) As (iii) but with a Rose criterion of 3. (vi) As (ii) but including errors in the phase retrieval algorithm (Hagemann & Salditt, 2017BB22) giving a factor of 37 increase in required fluence. (b) Dose. (i)–(vi) as in (a). (vii) As in (ii) but with dose distributed over a model cell (70% water) rather than confined to a protein.

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