Figure 2
Sketches of the (A) laser and detector box and the (B) optics box. Markers denote: (a) excitation laser and (b) STED laser, (c) acousto-optic modulators, (d) apertures, (e) adjustable mirrors, (f) glass fiber cable front end in the laser and detector box, (g) glass fiber cable back end in the optics box, (h) easySTED unit, (i)–(j) additional guiding mirrors, (k) STED microscope objective, (l) xyz-piezo stage, (m) tubus lens, (n) dichroic mirror, (o) position towards the photodiode (i.e. front end of the second glass fiber cable inside the optics box), (p) photodiode, (q) motorized flippable mirror, (r) epifluorescence-LED, (s) dichroic mirror/emission filter for full-field mode and (t) CCD camera. |