Figure 3
(a) Overview of the components. The STED microscope is transferred to the beamline on a wheeled cart and consists of a computer rack including the laser and detector box and the optics box. (b)–(c) Side and top view of the integrated setup at the sample recording positions. The optics box is installed left and anti-parallel to the X-ray beam axis. (d) Photograph of the integrated STED and X-ray setup during the first synchrotron beam time. (1) STED microscope rack, (2) glass fiber cable, (3) optics box, (4) STED microscope objective, (5) sample and motorized tower, (6) stepping motor for y-translation, (7) granite blocks, (8) safety box and bright-field illumination, (9) top-view video camera, (10) KB box, (11) waveguide and xyz-mount, (12) on-axis video camera, (13) evacuated tube, (14) X-ray detectors and bench. Adapted from Bernhardt et al. (2018). |