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Figure 1
(A) Sketch of the experimental setup. A tissue section is scanned with a microfocused or nanofocused synchrotron beam. For microfocusing at beamline ID13, ESRF, a transfocator equipped with compound refractive lenses is commonly used. The tissue is sandwiched between two polypropylene foils and several microlitres of buffer solution are added to keep the sample moist during the experiment. It is swept continuously along the x-axis and stepped along the y-axis through the X-ray focus, and diffraction patterns are collected at regular time intervals. A beamstop is used to block the primary (unscattered) beam. (B) Map of the integrated intensity of a tissue section described in detail by Nicolas et al. (2017aBB35). (C) Scan parameters that can be varied to minimize the detrimental effect of radiation damage.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 26| Part 4| July 2019| Pages 980-990
https://doi.org/10.1107/S1600577519006817
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