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Figure 2
Change of the myosin (1,1) reflection intensity with varying scan parameters and increasing dose. (A) Average scattering pattern from a scan with 5 µm line step, showing the (1,1) myosin reflection at approximately 0.28 nm−1. (B) Variation of the line step parameter showing a decay of the azimuthally integrated profiles. Each profile is fitted using a simple power law decay and a Gaussian. (C)–(F) The extracted peak intensity is shown for a variation of four scan parameters. Black lines serve as guides to the eye. A red circle in each graph marks the parameter value that was used in scans where one of the three other parameters was varied. (C) The exposure time has also been varied without scanning, such that a single spot was repeatedly exposed (loop scan), as shown in gray. For these data, the accumulated dose is shown as a second axis. Upon variation of the exposure time, the peak intensity has already completely vanished at 50 ms. (D) The variation of the length of a scan line has no effect on the reflection intensity. (E) The contrary can be seen for a variation of the line step. The peak intensity continuously increases until a plateau is reached at approximately 4 µm. (F) Increasing the step size also leads to an increase in reflection intensity.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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