view article
Figure 2
Titrations of in-house recombinant proteins trigger canonical TGFβ-1 signalling pathways at the expected levels. (a) Levels of SMAD phosphorylation for commercial mature TGFβ-1 (blue) and in-house heated LTGFβ-1 (gold). (b) Titration of LAP against a fixed amount of mature TGFβ-1 (10 pM). Data were fitted to a four-parameter logistic regression (4PL) to determine the EC50 and IC50 values. (a) The EC50 values were 23.2 and 3.46 pM for in-house heated LTGFβ-1 and commercial TGFβ-1, respectively. (b) The IC50 for LAP was 5.7 pM (0.57 molar ratio).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 26| Part 4| July 2019| Pages 967-979
https://doi.org/10.1107/S1600577519005113
Follow J. Synchrotron Rad.
Sign up for e-alerts
E-alerts
Follow J. Synchrotron Rad. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS