Figure 7
Circular-dichroism spectra show that the secondary structure of LAP is different when unbound and bound to TGFβ-1 (LTGFβ-1). (a) Experimental spectra of LTGFβ-1 (light red) and LAP (light blue) compared with theoretical spectra of the crystal structure (LTGFβ-1, dark blue; LAP domain, dark red) calculated with PDB2CD. (b) Top, representative fit using BeStSel of the secondary-structure population approximation (red) of experimental LAP (blue); bottom, the residuals. |