view article

Figure 2
Schematic diagram of nucleic acid X-ray footprinting experiments. RNA folding experiments are an example application of X-ray footprinting. These experiments involve exposing unfolded and folded RNA samples to a focused X-ray beam, which produces hydroxyl radicals that cleave the phospho­diester backbone of the RNA molecule. The resulting nucleic acid fragments are analysed using gel electrophoresis. Missing regions observed on the sequencing gel for the folded sample indicate regions that become protected, or inaccessible to solvent (i.e. hydroxyl-radical cleavage), upon the unfolded RNA molecule assuming its folded state.

Journal logoJOURNAL OF
ISSN: 1600-5775
Follow J. Synchrotron Rad.
Sign up for e-alerts
Follow J. Synchrotron Rad. on Twitter
Follow us on facebook
Sign up for RSS feeds