Figure 2
Diffraction efficiency (DE, the number of elastically scattered photons per MGy of absorbed dose), normalized to the value at 12.4 keV, as a function of incident beam energy for a protein crystal containing no atoms heavier than sulfur of the 91 kDa GH7 family cellobiohydrolase (PDB 5mcc) (Bury et al., 2017). DE was calculated using the program RADDOSE-3D with no photoelectron or fluorescent escape from the crystal. There is a 1.76-fold increase in DE at 35 keV compared with that at 12.4 keV. |