Figure 4
I/D [equations (2) and (3)] normalized to the value at 12.4 keV as a function of incident beam energy for a protein crystal of the 91 kDa GH7 family cellobiohydrolase (PDB 5mcc) (Bury et al., 2017) which contains no atoms heavier than sulfur. The dose was calculated using the program RADDOSE-3D with no photoelectron or fluorescent escape from the crystal. The higher the resolution of the reflection, the bigger the improvement in I/D on increasing the beam energy, with a 1.66-fold increase from 12.4 keV to 35 keV for 1 Å reflections. |