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![[Figure 1]](ay5539fig1mag.jpg)
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Figure 1
Schematic illustration of the experimental procedures and beamline setup. 48hpf zebrafish larvae were collected, fixed and immersed into 1% osmium tetroxide solution for 30 min. Then, samples were washed in 1% cacodylate solution. Next, samples were dehydrated, washed with cacodylate buffer, bathed for 30 min in 100% isopropanol and placed into a 1 mm glass capillary filled with 100% isopropanol (1). After microtomography, samples were dehydrated through ascending series of ethanol solution, cleared in xylol (two baths of 15 min each) and embedded in Paraplast Plus. Following a second microtomography (2), samples were immersed in xylol to remove the embedding medium, and then bathed in 100% acetone (1 h) for critical-point drying and measured for the third time (3). (b) Schematic view of the experimental setup for synchrotron-based X-ray microtomography. (1) X-rays from a synchrotron bending-magnet source illuminated the samples positioned in front of an indirect X-ray detector. (2) Projection images, acquired by a rotational scan, were used to reconstruct the tomographic slices (3). |
![[Figure 1]](ay5539fig1.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
