journal menu
![[Figure 3]](ay5539fig3mag.jpg)
|
|
Figure 3
Segmentation followed by morphological analyses of the lens and retina volume of 48hpf zebrafish larvae after being serially submitted to each sample-mounting condition. (a) 3D views of an eye of a single segmented 48hpf zebrafish larvae imaged after each sample-mounting condition. The lens is digitally segmented in light gray and the retina in purple. (b) The left-hand bar graph show a more expressive reduction in lens volume after paraffin embedding and the right-hand graph exhibits the reduction in total lens volume plotted as a percentage of the isopropanol condition (first sample-mounting strategy). (c) The left-hand bar graph shows a more expressive reduction in retina volume after paraffin embedding and the right-hand graph exhibits the reduction in total retina volumes plotted as a percentage of the isopropanol condition (first sample-mounting strategy). (d) The left-hand graph presents the normalized ratio (%) of lens volume/whole sample volume. The right-hand graph presents the normalized ratio (%) of retina volume/whole sample volume. Where presented, lines connect individual samples to show the individual alteration throughout the procedure. Values indicate the mean ± standard deviation. *P < 0.05: the difference between groups was statistically significant; ns: not significant. Data were analyzed by one-way ANOVA followed by Bonferroni post-tests. N = 4 samples. Scale (X × Y): 250 µm × 250 µm. |
![[Figure 3]](ay5539fig3.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
