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Figure 4
Morphological analyses of segmented heart volumes of 48hpf zebrafish larvae after being submitted to each sample-mounting condition (a) 3D views of the heart of a single segmented 48hpf zebrafish larvae imaged after each sample-mounting condition. The organ is digitally segmented in red. (b) The left-hand bar graph shows a more expressive reduction in heart volume after paraffin embedding and the right-hand graph shows the reduction in total heart volume plotted as a percentage of the iso­propanol condition (first sample-mounting strategy). (c) Normalized ratio (%) of heart volume/whole sample volume. Where presented, lines connect individual samples to shown individual alteration throughout the procedure. Values indicate the mean ± standard deviation. *P < 0.05: the difference between groups was statistically significant; ns: not significant. Data were analyzed by one-way ANOVA followed by Bonferroni post-tests. N = 4 samples. Scale (X × Y): 200 µm × 300 µm.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 26| Part 6| November 2019| Pages 2013-2023
https://doi.org/10.1107/S1600577519011299
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