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Figure 5
Tomographic slices and FSC of the same 48hpf zebrafish larva for each sample-mounting procedure. (a) Z-slice from the same larva imaged in iso­propanol (top panel), paraffin embedding (middle panel) and critical-point dried (bottom panel). Observe the better image quality shown by single cell limits on a representative z-slice for the critical-point-dried procedure. Letters indicate the following structures: L, lens; R, retina; H, heart. (b) FSC showing a crescent image resolution: iso­propanol > iso­propanol/paraffin > iso­propanol/paraffin/critical-point dried. The tilt angle ranges from 0° to 180°.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 26| Part 6| November 2019| Pages 2013-2023
https://doi.org/10.1107/S1600577519011299
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