Figure 2
(a) Schematic showing the assembly of the flow cell from its components. The mica windows are shown as blue lines. The thickness of the flow channel (X-ray path length) is 1.5 mm. (b) Photograph of the flow cell installed at the beamline. The flow channels can be seen illuminated by LEDs. Also visible are the vacuum beam path (aluminium tube in the middle), the WAXS nose cone (beige, on the left) and the camera lens (red). (c) Details of the fluid connections in the sample handler. For clarity, only two PCR tubes are shown. The blue flow paths are one-way only, while fluids in the green paths can flow in both directions. Two valves are used. V1 is a four-port, two-position valve. In the position shown, the syringe pump has access to one of the PCR tubes through the top channel in the flow cell. The syringe pump and bottom channel are ready to be washed and dried, depending on the position of the two-position selection valve V2. The center-to-center distance between neighboring channels is 4.5 mm. The smaller holes between the PCR tubes in each row are the blank holes described in the text; one is under the upstream injection needle connected to channel B in the figure. |