Figure 4
LD imaging using DUV fluorescence microscopy. Sei1Δ cells incubated with DPH stain were observed using DUV fluorescence microscopy at 275 nm and 340 nm excitations to obtain TRP/TYR autofluorescence (a, b) and LD localization (c, d), respectively. Merged images (e, f) were presented with TRP/TYR and DPH fluorescences as pink and blue signals, respectively. LD (white arrow), unidentified TRP/TYR fluorescent structure (grey arrow), vacuole (V). |