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Figure 7
(a) Reconstructed slice in the xy plane of the PTA-stained punch biopsy (Sample B) obtained by the parallel-beam illumination. (b) Pixel segmentation into three classes via ilastik. (c) 3D rendering of the segmented cells located within the islet of Langerhans by their manual selection. (d) Boundary boxes of the segmented cells. Each square marks one component. If the width of one component is larger than 20 pixels in one dimension, it is declared as false (red squares). Squares of components with the correct size are marked green. (e) Cell volume distribution of the selected cells [green marked in (d)]. Note that the median volume size of a single cell is 271 voxel. (f) Distribution of the cell sphericity Ψ of the selected cells [green marked in (d)]. Note that the median cell sphericity is 0.87. Scale bars in (a), (b), (c) and (d): 50 µm.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 27| Part 6| November 2020| Pages 1707-1719
https://doi.org/10.1107/S1600577520011327
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