X-ray tomographic reconstruction and segmentation pipeline for the long-wavelength macromolecular crystallography beamline at Diamond Light Source

Kazantsev, D.; Duman, R.; Wagner, A.; Mykhaylyk, V.; Wanelik, K.; Basham, M.; Wadeson, N.

doi:10.1107/S1600577521003453

Journal of Synchrotron Radiation Journal of
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Figure 11
Thaumatin reconstruction and segmentation. From top row to bottom: the result of iterative reconstruction using the ToMoBAR package, manual segmentation using Avizo, a combined segmentation using GMM (liquor, loop and vacuum), GeoDistance thresholding and RegionGrow for the crystal. One can see that the GeoDistance method under-segments the crystal while RegionGrow is closer to the manual segmentation, yet slightly smoother.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 28| Part 3| May 2021| Pages 889-901

https://doi.org/10.1107/S1600577521003453

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