Millisecond timescale reactions observed via X-ray spectroscopy in a 3D microfabricated fused silica mixer

Huyke, D.A.; Ramachandran, A.; Ramirez-Neri, O.; Guerrero-Cruz, J.A.; Gee, L.B.; Braun, A.; Sokaras, D.; Garcia-Estrada, B.; Solomon, E.I.; Hedman, B.; Delgado-Jaime, M.U.; DePonte, D.P.; Kroll, T.; Santiago, J.G.

doi:10.1107/S1600577521003830

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Figure 3
Fluorescein focusing and fluorescein–iodide quenching simulations and experiments in the fused silica chip region of the mixer. (a) Normalized fluorescence intensity fields at the y = 0 plane from simulations (left column) and confocal laser scanning experiments (right column). Shown is hydrodynamic focusing of a fluorescent sample by pure buffer (top row) and buffer with a quencher (bottom row). (b) Sample FWHM w_sa versus axial position x as predicted by simulations and experimentally measured. (c) Quenched intensity ratio versus x predicted by simulations and experimentally measured. We hypothesize the fluctuations in the quenched intensity ratio are due to nonuniform illumination and detection within the focusing region of the chip. Note that those fluctuations appear for all flow conditions and are discussed in Appendix C

. Data in (b) and (c) are for varied Q_sa and fixed Q_sh = 1 ml min⁻¹.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 28| Part 4| July 2021| Pages 1100-1113

https://doi.org/10.1107/S1600577521003830

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