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![[Figure 4]](ye5004fig4mag.jpg)
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Figure 4
Automated determination of the buffer subtraction scaling factor. The scattering data from a lysozyme sample are shown as an example. The progression of the scaling factor adjustment is shown as (a) weighted scattering intensity q × I (only data at q > 0.3 Å−1 are used) and (b) the span between the maximum and minimum for the buffer-subtracted data. The data in (b) are presented as a series of histograms. The color code is the same for (a) and (b). From top to bottom, the corresponding scaling factors used for buffer subtraction are 0.990, 0.993, 0.995, 0.996, 0.9963, 0.9966, 0.9969, 0.9972, 0.9975, 0.9978. The final subtraction result is shown in green in (c), together with the protein solution scattering (blue) and scaled buffer scattering (orange), using a scaling factor of 0.9970. The final scaling factor falls between two plotted values, as indicated by the red arrow in (b). |
![[Figure 4]](ye5004fig4.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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