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Figure 4
Scavengers exhibit concentration-dependent changes in integrated intensity. The effectiveness of each scavenger (ascorbic acid, cysteine, and sodium nitrate) to inhibit fragmentation was judged by comparing relative X-ray dose-driven changes in the accumulated integrated intensity (AII) from 24 Gy (orange) to 792 Gy (purple). This method provides the running total integrated intensity as the total X-ray dose increases. Column heights that show the smallest deviation from zero indicate the most effective damage protection. Each scavenger was tested at seven concentrations ranging from 50 nM to 50 mM in addition to the highest concentration of scavenger in the absence of protein (50 mM buffer). Each scavenger exhibited concentration-dependent effects. Low concentrations (50 nM–5 µM) increased the amount of fragmentation while higher concentrations (500 µM–5 mM) mitigated the damage. The effectiveness of each scavenger at a particular concentration also differed. The horizontal dashed line refers to the final AII value (1.35) of the control after 792 Gy.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 28| Part 5| September 2021| Pages 1309-1320
https://doi.org/10.1107/S1600577521004045
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