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Figure 6
Scavengers alter the kinetics of fragmentation. Three scavengers (a) ascorbic acid, (b) cysteine, and (c) sodium nitrate were tested for their abilities to mitigate fragmentation of the engineered di­sulfide bond. Each scavenger was tested at seven concentrations: 50 nM (red), 500 nM (dark blue), 5 µM (light blue), 50 µM (light purple), 500 µM (orange), 5 mM (brown), 50 mM (green) and compared with the X-ray dose-dependent integrated intensity trajectory of the control (no scavenger; yellow) and the 50 mM buffer (dark purple) of each respective scavenger. (Dashed lines) Scavengers at low concentrations exhibit exponential decays in integrated intensity that reflect a first-order process whereas at high concentrations the integrated intensity decay becomes linear reflecting a zeroth-order process.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 28| Part 5| September 2021| Pages 1309-1320
https://doi.org/10.1107/S1600577521004045
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