Figure 1
Photographs of the front (a) and rear (b) of the 96-well high-throughput apparatus mounted on a two-axis motion stage. In the front view, PCR tubes are shown installed into the copper sample block, with purple insulating material around the edges of the block to minimize heat absorption from the environment when the block is being cooled. The Kapton window for the front exposure side of the device is removable to facilitate sample loading and unloading. The rear panel of the unit (b) includes a nitrogen gas port, thermocouple connectors and the DC power connector for the Peltier coolers. Panel (c) shows an exploded view of the internal layers of the device to illustrate the assembly, in which the sample block that holds the 96 PCR tubes is stacked on top of a cooling plate that is in thermal contact with the Peltier coolers. These coolers are in turn attached to a water-cooling block for heat removal during Peltier operation. |