Hydroxyl radical mediated damage of proteins in low oxygen solution investigated using X-ray footprinting mass spectrometry

Kristensen, L.G.; Holton, J.M.; Rad, B.; Chen, Y.; Petzold, C.J.; Gupta, S.; Ralston, C.Y.

doi:10.1107/S1600577521004744

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Figure 1
Concentration dependence of X-ray damage. SDS-PAGE of cyt c (a) and BSA (b) after X-ray irradiation of 0–200 ms and for different protein concentrations. BSA concentrations were 2, 20, and 200 µM, while cyt c concentrations were 5, 20, and 200 µM. For reference, the calculated absorbed radiation dose by the buffer was 162 Gy for a 10 ms X-ray exposure and 3240 Gy for a 200 ms exposure.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 28| Part 5| September 2021| Pages 1333-1342

https://doi.org/10.1107/S1600577521004744

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