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Figure 2
Relative oxidation in air-saturated versus nitro­gen-equilibrated sample preparations. (a) SDS-PAGE of 20 µM cyt c in air-equilibrated and nitro­gen-equilibrated sample preparations, with X-ray exposure range of 0–100 ms. For reference, the calculated absorbed radiation dose by the buffer was 162 Gy for a 10 ms X-ray exposure to 3240 Gy for a 200 ms exposure. (b) MALDI spectra of cyt c in air (left) versus nitro­gen (right) sample preparations for the 100 ms samples, with inset of monomer peaks.

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