Figure 2
Integration of individually characteristic spectral regions: (A) the summation of the unit area normalized spectral functions used for linear least-squares fitting are summed into a single vector (orange) and the second derivative (blue) is taken to identify the inflection points (vertical dashed red lines). (B) The raw fluorescence from the mean spectrum from the peel (black) has been corrected by the blank spectrum (cyan) to yield the blank-corrected spectrum (red). All channels between each inflection point (vertical black lines) have been integrated into a single value (blue bars with the black circles), from the blank-corrected spectrum, to reduce the data set from 2048 channels to 55. |