Figure 2
Synchrotron WFDUV mapping of typical kidney biopsies. The signal (yellow) was obtained after subtraction of the 412–438 nm channel and 327–353 nm channel fluorescence map. The background signal (grey) was obtained using the 327–353 nm channel (aromatic amino-acid fluorescence). (A) Normal kidney. (B) Primary hyperoxaluria patients present large CaOx crystals (arrow), small punctiform structures compatible with microcrystals (asterisks) and a diffuse signal in the cytoplasm of tubular cells. (C) Secondary hyperoxaluria have either large deposits (arrow) and punctiform signals (asterisks) without clear tubular diffuse distribution or (D) only large deposits (arrow). |