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Figure 3
Complexometric titration of free Ca2+ in the remineralization solution using 17% EDTA. Note that the colorless remineralization solution turned purple when Eriochrome Black T formed a complex with unbound Ca2+ (a). However, when 200 µl of 17% EDTA was added, unbound Ca2+ formed a complex with EDTA instead and the solution hence immediately turned blue as indicated by Eriochrome Black T. The saturation point of dark blue color indicates the absence of free Ca2+ available in the remineralization solution (b).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 29| Part 2| March 2022| Pages 496-504
https://doi.org/10.1107/S1600577521013655
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