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Figure 6
Virtual sectioning of a poplar stem by tomography based on X-ray scattering contrast. (a) Assuming the structures in the sample exhibit a fiber geometry, with the fiber axis perpendicular to the incident X-ray beam, we reduce all scattering patterns to 1D intensity profiles (blue line) by azimuthal averaging. These data are further processed by smoothing (orange line) to eliminate sharp features caused by the non-cellulose components in the sample. (b) For this experiment, we select the intensity under the cellulose (200) peak at q = 1.58 Å−1 and the intensity near q = 2.27 Å−1 to represent the abundance of crystalline cellulose and amorphous components, respectively, as indicated by the blue and green boxes in (a). Sinograms are generated based on the estimated material abundance. The crystalline content sinogram is shown as an example. (c) Several tomograms are derived from the data using tomopy, based on the abundance of (A) crystalline cellulose and (B) amorphous components. (C) is the ratio of (A) over (B), similar to the crystalline index customarily used in cellulose research. For comparison, the tomogram based on absorption contrast is shown in (D).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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